Varying opinions on the authenticity of a human midgut carcinoid cell line--letter.

• In 2007, our laboratory published an article in Clinical ncer Research reporting on the development of a human idgut carcinoid tumor cell line (1). This cell line has now en shared with over 20 laboratories around the world. vestigators in the field of neuroendocrine tumor rearch are aware that there are very few human carcinoid ll lines available for use in vitro and for murine studies. the time of publication of this article, we believed that e had developed the first human midgut carcinoid cell e in North America. These cells expressed dense neurocretory granules on electron microscopy, VMAT-1/2, and matostatin receptors (1). Recently, we performed short ndem repeat genotyping of the CNDT cell line, and we ere unable to match this cell line to any other cell line in r own database at M.D. Anderson Cancer Center, The hn Hopkins database, or the American Type Culture llection database. This, in some ways, was reassuring these databases contain data on thousands of cell lines, d we could rule out contamination of our CNDT2 cells other well-characterized cell lines. However, we then ed to match our cell line with frozen carcinoid specimens our tissue bank, and unfortunately, we were unable to atch our short tandem repeat genotype to the carcinoid mor that we thought was the source of this cell line. e short tandem repeat data for CNDT2 is listed below: for these indolent carcinoid cell lines. As a result,